This book describes the concepts underlying the process of protein purification. The strategies and equipment needed in the protein purification was described. Advanced techniques like Polyacrylamide Gel Electrophoresis and Mass spectroscopy were explained. Isolation of proteins through protein chromatography was illustrated. Some of the advanced platforms such as proteomics and associated areas were described in detail. The sophisticated instrumentation of HPLC in the analysis and purification of proteins was explained in detail. Different types of electrophoresis were discussed. The role of nanotechnology in the protein purification process was explained. The analysis of proteins and purification technology were emphasized in detail for a systematic study.